Talk:Reverse transcription polymerase chain reaction

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Molecular Biology: Genetics / MCB

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This article was the subject of a Wiki Education Foundation-supported course assignment, between 25 January 2021 and 20 May 2021. Further details are available on the course page. Student editor(s): Phlebbie.

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Untitled

RT PCR is also short for real time PCR

Real time PCR is not a variation of reverse transcriptase PCR AFAIK...Its simply a PCR reaction where the components are tracked in real time... Jarwulf 01:34, 31 Mar 2005 (UTC)

It seems to me that qRT-PCR (also RT-PCR, real time PCR, etc.) is indeed a type of Reverse transcription PCR. With 'vanilla' or 'plain' RT-PCR once simply amplifies RNA transcripts by PCR by simply using an initial reverse transcription step. In qRT-PCR, we also do an initial reverse transcription step, but the difference is that the increased amplification of the PCR product is tracked at the various cycles of the PCR, thus providing a quantification of how much RNA was in the original sample. Thus real time PCR is just reverse transcription PCR tracked trough time. Because qRT-PCR has become the standard, real time PCR and reverse transcriptase PCR have become almost synonymous. In reality there seems to be little reason to do a plain RT-PCR, as we could not quantify the amount of RNA in the original sample. The article is confusing in extreme as it is written. 114.112.239.243 (talk) 10:34, 15 November 2015 (UTC)[reply]

Reverse transcription PCR is not to be confused with real-time polymerase chain reaction (Q-PCR/qRT-PCR), which is also sometimes (incorrectly) abbreviated as RT-PCR. Sorry, but this is not correct. Everyone says RT-PCR when really they mean qRT-PCR. I was just searching in Pubmed Central (150k biomedical research articles). The word qRT-PCR appears 13 times, Q-PCR appears once and RT-PCR/RTPCR/"RT PCR" appear ~1400 times. It is not 'sometimes' incorrectly abbreviated, it is a very common abbreviation, which might not be exactly correct, but, heck, this is biology... there are tons of terms that are not really defined... --Maximilianh (talk) 11:41, 19 November 2009 (UTC)[reply]

Is this really a stub anymore? - Orborde 04:57, 7 May 2005 (UTC)[reply]

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"fitst" should be "first" - AZ - Jan 6, 2006

Removed Link Directory Spam Bioinformin Bold text

Reverse transcriptase PCR needs to be redirected here.Jasoninkid 19:02, 11 May 2007 (UTC)[reply]

RTPCR needs to be a disabiguty page not a redirect. Jasoninkid 18:40, 2 November 2007 (UTC)[reply]

When was this technique develpoed, and when did it start to become comon? -OOPSIE- (talk) 07:26, 6 December 2007 (UTC)[reply]

I want to do a complete overhaul...

RT-PCR is a very commonly used technique in molecular biology (or at least it's one i'm very familliar with), and I want to do a complete overhaul of this page. There are too many typos, too many misconceptions, and too many omissions of the most important parts. I just started today, but over the coming days, I want to make big changes. Feel free to discuss anything with me. Thanks. --Michael (talk) 18:13, 28 February 2008 (UTC)[reply]

Most Rt enzymes including the most commonly used are not most active at 37 but 42 degrees

Is there a misconception from the first step to the second step mentioned here in this page? here it should be talking about a DNA-RNA complex rather than a dsDNA after the action of reverse transcriptase, and the follow up process should involve a RNase to degenerate the RNA component and should be followed by the normal PCR procedure.

I am not an experienced laboratory technician, please correct me if I am wrong.

Yanij

—Preceding unsigned comment added by Yanij (talk • contribs) 19:31, 9 November 2009 (UTC)[reply]

Yanij, yea it should be RNA/ DNA, i made the edits.

To Future editors: This is the Reverse transcription PCR page not the Real time PCR page be wary when making edits. I just removed a lot of Real Time PCR information. — Preceding unsigned comment added by Eleten (talk • contribs) 04:03, 8 December 2011 (UTC)[reply]

Plans for contents addition

Hi all. A group of us will be adding new contents to this article over next several weeks. Our plan is to organize the article into following seven sections:

Introduction
History
Principles
Application
Challenges
Protocol
Publication Guidelines

Your suggestions, comments, and edits are always welcomed and appreciated. Kkotani1 (talk) 01:44, 14 November 2012 (UTC)[reply]

Final week mini-review

Hi, guys. Here's a final-week mini-review of your page. It is just a few random notes, that I jotted down while skimming the page, and is not intended as a comprehensive review.

It would be nice if you could add some figures. Please see some suggestions here, under "Figures and Images".
"is often interchanged with": do you mean "is often confused with"?
Under "History", you have some content that seems to have nothing to do with history. For example, the last paragraph of that section. Perhaps this content could be moved to some other section(s) where it would fit better?
Under "Principles" you have the same problem, in the paragraph beginning, "Despite its major advantages, RT-PCR is not without drawbacks." Perhaps you could have a "pros and cons" subsection at the end of "Principles", and move these there -- it is just a thought, other solutions might be better.
In general, your article is pretty good, and is among the best from this class that I've looked at so far. You might look to an article GA-rated article like X-ray_crystallography for some ideas, if you want to restructure it a little bit during this last week.
Under "Application", I think each of the paragraphs that have a bold inline-header, should really be a subsection. That way, they'll show up in the table of contents.

Klortho (talk) 18:45, 9 December 2012 (UTC)[reply]

Thanks for the suggestions! How do I go about making each paragraph that has a bold inline-header in the "Abbplication" section a subsection?MHN2785 (talk) 16:37, 12 December 2012 (UTC)[reply]

Boghog already fixed that in this edit. See Wikipedia:Layout#Headings_and_sections. Adrian J. Hunter^{(talk•contribs)} 00:13, 13 December 2012 (UTC)[reply]

Assessment comment

The comment(s) below were originally left at Talk:Reverse transcription polymerase chain reaction/Comments, and are posted here for posterity. Following several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section.

is the process of Real time PCR similar to the Reverse transcriptase PCR?

Last edited at 23:09, 15 February 2007 (UTC). Substituted at 04:14, 30 April 2016 (UTC)

rRT-PCR=qRT-PCR

rRT-PCR, real time RT-PCR. is used in Coronavirus_disease_2019. rRT-PCR appears to be the contemporary (2020) usage and preferable to RRT-PCR. The mixed case, rRT-PCR, usage needs to be included in Nomenclature. LarryLACa (talk) 21:08, 3 April 2020 (UTC)[reply]

Added cite for Covid-19 rRT-PCR usage in Nomenclature LarryLACa (talk) 04:39, 4 April 2020 (UTC)[reply]

"... monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR)." certainly suggests rRT-PCR = qRT-PCR. Whereas the next sentence, "Combined RT-PCR and qPCR are routinely used ...", suggests rRT-PCR and qRT-PCR are distinct. The next paragraph remarks "Such use may be confusing, ... ." Well yah, with a little imagination and effort jargon can cause confusion. Conversely, a lucid and honest explanation makes a concept evident and prevents confusion. Regards, ... PeterEasthope (talk) 02:23, 11 April 2020 (UTC)[reply]